LOCUS       sequence-89403-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Cofilin-N-10
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..384)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(175..196)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     524..543
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     783..804
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
       61 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt
      121 cgcggccgct tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      181 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactgggt
      241 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      301 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcgggtct tgaagttcac
      361 cttgatgccg ttcttctgct tgtcggcggt gatatagacc ttgtggctgt tgtagttgta
      421 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      481 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      541 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
      601 gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac tgcacgccgt aggtcaaggt
      661 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
      721 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
      781 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgcc
//
