LOCUS       sequence-89407-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivector that expresses LifeAct-GFP from internal human PGK 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  TITLE     Lentivirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti.PGK.LifeAct-GFP.W
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..135
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      151..160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(157..159,160..162,163..675)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(202..223)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(463..482)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     810..831
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cctcgttgac cgaatcaccg acctctctcc ccagggggat cctagcgcta ccggactcag
       61 atctcgagct caagcttcga attcatgggt gtcgcagatt tgatcaagaa attcgaaagc
      121 atctcaaagg aagaagggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
      181 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      241 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      301 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
      361 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      421 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      481 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      541 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      601 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
      661 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      721 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
      781 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      841 gggatcactc tcggcatgga cgagc
//
