LOCUS       sequence-89408-e        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human L-MYC and LIN28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hUL
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
       61 tggttattgt gctgtctcat cattttggca aagaattcgc ccttcaccat ggactacgac
      121 tcgtaccagc actatttcta cgactatgac tgcggggagg atttctaccg ctccacggcg
      181 cccagcgagg acatctggaa gaaattcgag ctggtgccat cgccccccac gtcgccgccc
      241 tggggcttgg gtcccggcgc aggggacccg gcccccggga ttggtccccc ggagccgtgg
      301 cccggagggt gcaccggaga cgaagcggaa tcccggggcc actcgaaagg ctggggcagg
      361 aactacgcct ccatcatacg ccgtgactgc atgtggagcg gcttctcggc ccgggaacgg
      421 ctggagagag ctgtgagcga ccggctcgct cctggcgcgc cccgggggaa cccgcccaag
      481 gcgtccgccg ccccggactg cactcccagc ctcgaagccg gcaacccggc gcccgccgcc
      541 ccctgtccgc tgggcgaacc caagacccag gcctgctccg ggtccgagag cccaagcgac
      601 tcggagaatg aagaaattga tgttgtgaca gtagagaaga ggcagtctct gggtattcgg
      661 aagccggtca ccatcacggt gcgagcagac cccctggatc cctgcatgaa gcatttccac
      721 atctccatcc atcagcaaca gcacaactat gctgcccgtt ttcctccaga aagctgctcc
      781 caagaagagg cttcagagag gggtccccaa gaagaggttc tggagagaga tgctgcaggg
      841 gaaaaggaag atgaggagga tgaagagatt gtgagtcccc cacctgtaga aagtgaggct
      901 gcccagtcct gccaccccaa acctgtcagt tctgatactg aggatgtgac caagaggaag
      961 aatcacaact tcctggagcg caagaggcgg aatgacctgc gttcgcgatt cttggcgctg
     1021 agggaccagg tgcccaccct ggccagctgc tccaaggccc ccaaagtagt gatcctaagc
     1081 aaggccttgg aatacttgca agccctggtg gggg
//
