LOCUS       sequence-89410-e         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTriex-based expression vector which adds a C-his8 tag to the 
            inserted gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  TITLE     OPPF vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOPINEneo
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    67..529
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     456..473
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tgcatccatc ttgttcaatg gccgatccca tattggctgc aggaattatc gcgggttgtg
       61 gcaagcttat catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag
      121 acgttttttt aacctcgact aaacacatgt aaagcatgtg caccgaggcc ccagatcaga
      181 tcccatacaa tggggtacct tctgggcatc cttcagcccc ttgttgaata cgcttgaggt
      241 gagccatttg actctttcca caactatcca actcacaacg tggcactggg gttgtgccgc
      301 ctttgcaggt gtatcttata cacgtggctt ttggccgcag aggcacctgt cgccaggtgg
      361 ggggttccgc tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag aagcttccag
      421 aggaactgct tccttcacga cattcaacag accttgcatt cctttggcga gaggggaaag
      481 acccctagga atgctcgtca agaagacagg gccaggtttc cgggccctca cattgccaaa
      541 agacggcaat atggtggaaa ataaccggaa ttaccacacc agccaccacc ttctgatagg
      601 cagcctgcac ctgaggttaa tcact
//
