LOCUS       sequence-89412-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTirex based GFP fusion vector for expression in mammalian, E. coli 
            and insect cells using the baculovirus system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  TITLE     OPPF vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOPINEneo-3C-GFP
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(117..140)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(147..179)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(263..286)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(332..353)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     681..700
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa taaccggaat
       61 taccacacca gccaccacct tctgataggc agcctgcacc tgaggttaat cacttagtga
      121 tggtgatggt gatggtggtg ctcgagatcc tcggggtctt ccggggcgag ttctggctgg
      181 cttgcacgtg tatacagctg tgcggccgca agcttcgaac cggtaccgtc gacctgcagg
      241 cgcgccgaga tctgagaatt cagtgatggt gatggtgatg gtgatgtttc ttgtacagct
      301 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
      361 gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca
      421 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
      481 tgccgtcctc gatgttgtgg cgggtcttga agttcacctt gatgccgttc ttctgcttgt
      541 cggcggtgat atagaccttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
      601 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
      661 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
      721 cctggacgta gccttcgggc atggcggact tgaagaagtc
//
