LOCUS       sequence-89420-e        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal chromosomal tagging with stable GFP under control of GAL 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  TITLE     Pringle Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(79..276)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     1005..1024
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ggccactagt ggatctgata tcatcgatga attcgagctc gtttaaactg gatggcggcg
       61 ttagtatcga atcgacagca gtatagcgac cagcattcac atacgattga cgcatgatat
      121 tactttctgc gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata
      181 aatcacgcta acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga
      241 caagttcttg aaaacaagaa tctttttatt gtcagtactg attagaaaaa ctcatcgagc
      301 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
      361 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
      421 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
      481 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
      541 aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
      601 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
      661 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
      721 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
      781 gctgttttgc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
      841 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
      901 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
      961 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     1021 tacc
//
