LOCUS       sequence-89423-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal chromosomal tagging with stable GFP under control of GAL 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  TITLE     Pringle Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(37..184)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     promoter        279..720
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    279..396
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     691..714
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     complement(783..811)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tggaccagaa ctacctgtga aattaataac agacatactc caagctgcct ttgtgtgctt
       61 aatcacgtat actcacgtgc tcaatagtca ccaatgccct ccctcttggc cctctccttt
      121 tcttttttcg accgaattaa ttcttaatcg gcaaaaaaag aaaagctccg gatcaagatt
      181 gtacagatct gtaaagagcc ccattatctt agcctaaaaa aaccttctct ttggaacttt
      241 cagtaatacg cttaactgct cattgctata ttgaagtacg gattagaagc cgccgagcgg
      301 gtgacagccc tccgaaggaa gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc
      361 ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca ataaagattc tacaatacta
      421 gcttttatgg ttatgaagag gaaaaattgg cagtaacctg gccccacaaa ccttcaaatg
      481 aacgaatcaa attaacaacc ataggatgat aatgcgatta gttttttagc cttatttctg
      541 gggtaattaa tcagcgaagc gatgattttt gatctattaa cagatatata aatgcaaaaa
      601 ctgcataacc actttaacta atactttcaa cattttcggt ttgtattact tcttattcaa
      661 atgtaataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa cgtcaaggag
      721 aaaaaacccg gatctcaaaa tgtctttaat taacagtaaa ggagaagaac ttttcactgg
      781 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa tttttctgtc
      841 cagtggaaga gggtgaaagg tgatgcaaca tacggaaaaa cttacccctt taaaatttat
      901 ttggcactaa ctggaaaaac tacctggttc catgggccaa ca
//
