LOCUS       sequence-89428-e         560 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTriex-based vector with signal sequence and N-his tag for protein 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 560)
  TITLE     OPPF vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOPINH
FEATURES             Location/Qualifiers
     source          1..560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     91..110
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        91..109
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    110..134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        150..259
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             370..387
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    461..482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(497..502,503..520,521..527)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    535..551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
       61 ttttggcaaa gaattggatc ggaccgaaat taatacgact cactataggg gaattgtgag
      121 cggataacaa ttccccggag ttaatccggg acctttaatt caacccaaca caatatatta
      181 tagttaaata agaattatta tcaaatcatt tgtatattaa ttaaaatact atactgtaaa
      241 ttacatttta tttacaatca aaggagatat accatgggga tccttcccag ccctgggatg
      301 cctgcgctgc tctccctcgt gagccttctc tccgtgctgc tgatgggttg cgtagctgaa
      361 accggcgcac accatcacca ccatcacagc agcggtctgg aagttctgtt tcagggtacc
      421 gtcgacccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      481 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      541 gcggataaca atttcacaca
//
