LOCUS       sequence-89430-e         553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTirex based GFP fusion vector for expression in mammalian, E. coli 
            and insect cells using the baculovirus system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 553)
  TITLE     OPPF vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOPINN-GFP
FEATURES             Location/Qualifiers
     source          1..553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     85..104
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        85..103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    104..128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        144..253
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             274..291
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(334..355)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
       61 caaagaattg gatcggaccg aaattaatac gactcactat aggggaattg tgagcggata
      121 acaattcccc ggagttaatc cgggaccttt aattcaaccc aacacaatat attatagtta
      181 aataagaatt attatcaaat catttgtata ttaattaaaa tactatactg taaattacat
      241 tttatttaca atcaaaggag atataccatg gcacaccatc accaccatca cgtgagcaag
      301 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      361 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      421 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      481 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      541 ttcaagtccg cca
//
