LOCUS       sequence-89431-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTirex based GFP fusion vector for expression in mammalian, E. coli 
            and insect cells using the baculovirus system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  TITLE     OPPF vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOPINN-GFP
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(81..254)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     203..225
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     217..234
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     218..234
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(250..266)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(250..266)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(263..285)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    274..290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(298..304,305..322,323..328))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    343..364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(480..501)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tggccacacc agccaccacc ttctgatagg cagcctgcac ctgaggttaa tcacaaactg
       61 gtctagaaag cttggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
      121 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
      181 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaatcc
      241 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      301 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      361 attaattgcg ttgcgctcac tgcccgcttt ccagtcgggt cgacggtacc ctgaaacaga
      421 acttccagac cgctgctctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
      481 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
      541 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
      601 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
      661 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
      721 ttgtact
//
