LOCUS       sequence-89439-e         447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-replicating episomal expression of EBNA1, which enhances 
            transfection efficiency and expression from episomal plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     Sequence Label: pCXWB-EBNA1
FEATURES             Location/Qualifiers
     source          1..447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
       61 gttattgtgc tgtctcatca ttttggcaaa gaattcgccc ttgtcgacac catgtctgac
      121 gaggggccag gtacaggacc tggaaatggc ctaggagaga agggagacac atctggacca
      181 gaaggctccg gcggcagtgg acctcaaaga agagggggtg ataaccatgg acgaggacgg
      241 ggaagaggac gaggacgagg aggcggaaga ccaggagccc cgggcggctc aggatcaggg
      301 ccaagacata gagatggtgt ccggagaccc caaaaacgtc caagttgcat tggctgcaaa
      361 gggacccacg gtggaacagg agcaggagca ggagcgggag gggcaggagc aggaggggca
      421 ggagcaggag gaggggcagg agcagga
//
