LOCUS       sequence-89441-e        1332 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the membrane targeted Y-GECO1 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, 
            Campbell RE, Harrison DJ
  TITLE     Microfluidic cell sorter-aided directed evolution of a protein-based
            calcium ion indicator with an inverted fluorescent response.
  JOURNAL   Integr Biol (Camb). 2014 May 19.
  PUBMED    24840546
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Integr Biol
            (Camb). 2014 May 19."
COMMENT     Sequence Label: AAV-hSyn-Lyn-Y-GECO1
FEATURES             Location/Qualifiers
     source          1..1332
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1092..1106
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 atgggatgta taaagagtaa aagaaaagat aacttaaatg atgacgaaga tgataaggat
       61 ctcgccacaa tggccgactc atcacgtcgt aagtggaata agacacacgc agtcagagct
      121 ataggccggc tgagctcact cgagggttat agtaccgaga aaattgtgcc tgttccaaag
      181 caagggatat taaaaggaaa ggtcagcatg tacctattgc tgaaggatgg cggtcgttat
      241 cattgccagt ttgatacggt atataaagct aaatcagtgc cctccaagat gccaggacgg
      301 cattttatac agcataagct ccttagggag gaccgctctg atgctaaaaa ccataaatgg
      361 caattaacag aacatgcaat cgcaggcatg gacgagctgt acaagggagg tacaggcggg
      421 agtatggtga gcaagggcga ggggcaatcg aagcatgggc taaaggagga gatgacggtg
      481 aagtaccaca tggaaggttg cgttaatggt cacaaatttg tcattactgg agagggcatt
      541 ggaaaccctt ttaagggtaa gcaaactgca aatttgtgtg tgatagaagg aggcccgctg
      601 ccgttctcgg aggacatttt aagtccgggt tttaaatatg gtgaccggat tttcacagag
      661 tacccacagg atattgtaga ttacttcaag aactcatgtc cagagggcta tacgtgggaa
      721 aggagcttct tctatgagga cggagcggtc tgtcgatgca acgcggacat aacagtctct
      781 gaaaaggaga actgcatcta tcacaaaagt atcttctatg gggtgaattt tcccgccgac
      841 ggccccgtaa tgaagaaaat gaccactaat tgggaagctg acacgcgtga ccaactgact
      901 gaagagcaga tcgcagaatt taaagaggct ttctccctat ttgacaagga cggggatggg
      961 acgatgacaa ccgaggagct ggggacggtg ctgcggtctc tggggcagaa cccctcagaa
     1021 gcagagctgc gggacatgat caatgaagta gatgccgacg gtgacggcac aatcggcttc
     1081 cctgagttcc tgacgatgat gacaagaaaa atgaatgaca cggacagtga agaggaaatt
     1141 agagaagcgt tccgtgtgtt tgataaggac ggcaatggct acatcggcgc agcagagctt
     1201 cgccacgtga tgacaaacct tggagagaag ttaacagatg aagagtttga tgaaatgatc
     1261 agggtagcag actccgacgg ggatggtcag gtaaactacg aagagtttgt acaaatgatg
     1321 acagcaaagt ga
//
