LOCUS       sequence-89443-e        1368 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encoded a yellow fluorescent calcium ion indicator with inverted 
            fluorescence response for expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, 
            Campbell RE, Harrison DJ
  TITLE     Microfluidic cell sorter-aided directed evolution of a protein-based
            calcium ion indicator with an inverted fluorescent response.
  JOURNAL   Integr Biol (Camb). 2014 May 19.
  PUBMED    24840546
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Integr Biol
            (Camb). 2014 May 19."
COMMENT     Sequence Label: CMV-Y-GECO1
FEATURES             Location/Qualifiers
     source          1..1368
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..63
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             118..174
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     CDS             1128..1142
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 atgggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg
       61 ggtcgggatc tgtacgacga agatgataag gatctcgcca caatggccga ctcatcacgt
      121 cgtaagtgga ataagacagg tcacgcagtc agagctatag gccggctgag ctcactcgag
      181 ggttatagta ccgagaaaat tgtgcctgtt ccaaagcaag ggatattaaa aggaaaggtc
      241 agcatgtacc tattgctgaa ggatggcggt cgttatcatt gccagtttga tacggtatat
      301 aaagctaaat cagtgccctc caagatgcca ggacggcatt ttatacagca taagctcctt
      361 agggaggacc gctctgatgc taaaaaccat aaatggcaat taacagaaca tgcaatcgca
      421 ggcatggacg agctgtacaa gggaggtaca ggcgggagta tggtgagcaa gggcgagggg
      481 caatcgaagc atgggctaaa ggaggagatg acggtgaagt accacatgga aggttgcgtt
      541 aatggtcaca aatttgtcat tactggagag ggcattggaa acccttttaa gggtaagcaa
      601 actgcaaatt tgtgtgtgat agaaggaggc ccgctgccgt tctcggagga cattttaagt
      661 ccgggtttta aatatggtga ccggattttc acagagtacc cacaggatat tgtagattac
      721 ttcaagaact catgtccaga gggctatacg tgggaaagga gcttcttcta tgaggacgga
      781 gcggtctgtc gatgcaacgc ggacataaca gtctctgaaa aggagaactg catctatcac
      841 aaaagtatct tctatggggt gaattttccc gccgacggcc ccgtaatgaa gaaaatgacc
      901 actaattggg aagctgacac gcgtgaccaa ctgactgaag agcagatcgc agaatttaaa
      961 gaggctttct ccctatttga caaggacggg gatgggacga tgacaaccga ggagctgggg
     1021 acggtgctgc ggtctctggg gcagaacccc tcagaagcag agctgcggga catgatcaat
     1081 gaagtagatg ccgacggtga cggcacaatc ggcttccctg agttcctgac gatgatgaca
     1141 agaaaaatga atgacacgga cagtgaagag gaaattagag aagcgttccg tgtgtttgat
     1201 aaggacggca atggctacat cggcgcagca gagcttcgcc acgtgatgac aaaccttgga
     1261 gagaagttaa cagatgaaga gtttgatgaa atgatcaggg tagcagactc cgacggggat
     1321 ggtcaggtaa actacgaaga gtttgtacaa atgatgacag cgaagtaa
//
