LOCUS       sequence-89445-e        1365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encoded a yellow fluorescent calcium ion indicator with inverted 
            fluorescence response for expression in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, 
            Campbell RE, Harrison DJ
  TITLE     Microfluidic cell sorter-aided directed evolution of a protein-based
            calcium ion indicator with an inverted fluorescent response.
  JOURNAL   Integr Biol (Camb). 2014 May 19.
  PUBMED    24840546
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Integr Biol
            (Camb). 2014 May 19."
COMMENT     Sequence Label: pTorPE-Y-GECO1
FEATURES             Location/Qualifiers
     source          1..1365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             28..60
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             115..171
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     CDS             1125..1139
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
       61 cgggatctgt acgacgaaga tgataaggat ctcgccacaa tggccgactc atcacgtcgt
      121 aagtggaata agacaggtca cgcagtcaga gctataggcc ggctgagctc actcgagggt
      181 tatagtaccg agaaaattgt gcctgttcca aagcaaggga tattaaaagg aaaggtcagc
      241 atgtacctat tgctgaagga tggcggtcgt tatcattgcc agtttgatac ggtatataaa
      301 gctaaatcag tgccctccaa gatgccagga cggcatttta tacagcataa gctccttagg
      361 gaggaccgct ctgatgctaa aaaccataaa tggcaattaa cagaacatgc aatcgcaggc
      421 atggacgagc tgtacaaggg aggtacaggc gggagtatgg tgagcaaggg cgaggggcaa
      481 tcgaagcatg ggctaaagga ggagatgacg gtgaagtacc acatggaagg ttgcgttaat
      541 ggtcacaaat ttgtcattac tggagagggc attggaaacc cttttaaggg taagcaaact
      601 gcaaatttgt gtgtgataga aggaggcccg ctgccgttct cggaggacat tttaagtccg
      661 ggttttaaat atggtgaccg gattttcaca gagtacccac aggatattgt agattacttc
      721 aagaactcat gtccagaggg ctatacgtgg gaaaggagct tcttctatga ggacggagcg
      781 gtctgtcgat gcaacgcgga cataacagtc tctgaaaagg agaactgcat ctatcacaaa
      841 agtatcttct atggggtgaa ttttcccgcc gacggccccg taatgaagaa aatgaccact
      901 aattgggaag ctgacacgcg tgaccaactg actgaagagc agatcgcaga atttaaagag
      961 gctttctccc tatttgacaa ggacggggat gggacgatga caaccgagga gctggggacg
     1021 gtgctgcggt ctctggggca gaacccctca gaagcagagc tgcgggacat gatcaatgaa
     1081 gtagatgccg acggtgacgg cacaatcggc ttccctgagt tcctgacgat gatgacaaga
     1141 aaaatgaatg acacggacag tgaagaggaa attagagaag cgttccgtgt gtttgataag
     1201 gacggcaatg gctacatcgg cgcagcagag cttcgccacg tgatgacaaa ccttggagag
     1261 aagttaacag atgaagagtt tgatgaaatg atcagggtag cagactccga cggggatggt
     1321 caggtaaact acgaagagtt tgtacaaatg atgacagcaa agtga
//
