LOCUS       sequence-89448-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of alpha3D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Walsh ST, Cheng H, Bryson JW, Roder H, DeGrado WF
  TITLE     Solution structure and dynamics of a de novo designed three-helix 
            bundle protein.
  JOURNAL   Proc Natl Acad Sci U S A. 1999 May 11;96(10):5486-91.
  PUBMED    10318910
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1999-05-11"; volume: "96(10)"; pages: 
            "5486-91"
COMMENT     Sequence Label: pET16b-alpha3D
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             14..36
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(314..332)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      328..375
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(550..555,556..572,573..578))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(605..623)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        691..795
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctctagaat aattttgttt aactttaaga aggagatata ccatgggttc ttgggctgaa
       61 ttcaaacaac gtctggcggc gatcaaaacc cgtctgcaag cgctgggtgg ttctgaagca
      121 gagctggcag catttgagaa agaaatcgcg gctttcgagt ccgaactgca ggcttataaa
      181 ggtaaaggta acccggaagt tgaagctctg cgtaaagaag ctgctgctat ccgtgacgag
      241 ctccaagcat accgtcacaa ctaggatccg gctgctaaca aagcccgaaa ggaagctgag
      301 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      361 ttgaggggtt ttttgctgaa aggaggaact atatccggat atcccgcaag aggcccggca
      421 gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga
      481 tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata
      541 aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
      601 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      661 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      721 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      781 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      841 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      901 gatcagttgg
//
