LOCUS       sequence-89449-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR 
            experiments. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello
            CC
  TITLE     A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2014 May 30. pii: genetics.114.166389.
  PUBMED    24879462
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 May 30. pii: genetics.114.166389."
COMMENT     Sequence Label: pCCM935
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..500
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans K09B11.12 U6 snRNA gene 
                     (Friedland et al., 2013)"
     misc_RNA        521..596
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    604..840
                     /label=3' CeU6
                     /note="region downstream of the C. elegans K09B11.12 U6 
                     snRNA gene"
ORIGIN
        1 ctccaagaac tcgtacaaaa atgctctgaa gtaggtctcg agatcaatac tgggaagacg
       61 aaagtcttgc gaaaccgatt cgctgacccc agtaaagtct acttcggtag cccttccccc
      121 accacccagc tcgacgacgt cgacgagtac atctacctcg gtcgtcaaat caacgcccaa
      181 aacaacttga tgccggaaat ccaccgaaga cgtcgagcag cctgggctgc attcaatgga
      241 atcaagaatg ccaccgactc catcaccgac aagaagattc gtgcgaatct gttcgactca
      301 attgtccttc cagcgctcac ctacgtttca gaagcctgga cattcaccaa agctctatcc
      361 gaacgagtac gaatcacaca tgcctccccc acaaattatt gatctacatc cccatttggt
      421 atagtgtctt acaggactct caatatgtca gctgcatgat aaatggtccc tatataattg
      481 aattgcaaat ctaaatgttt gaacccgttg ccgaatacac gttttagagc tagaaatagc
      541 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
      601 tttcaataat attctagata tccctttttg aaactaaaat gaaatactct aaatttttga
      661 gttcaatgtt tcaactaatc ttggttaaag gtaaattccc ggagggacga ttttaaagtt
      721 gccagagttg attaaattgg tccaaaaaat tttattagga aactcgaaaa aaaattgatt
      781 ttttttgaca atattccaaa gaaaattaca aaatctcgac gccaaacata gtcggctgtg
//
