LOCUS       sequence-89450-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 
            (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a
            4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B
            for FRET..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Degtyar V, Hafez IM, Bray C, Zucker RS
  TITLE     Dance of the SNAREs: assembly and rearrangements detected with FRET 
            at neuronal synapses.
  JOURNAL   J Neurosci. 2013 Mar 27;33(13):5507-23. doi: 
            10.1523/JNEUROSCI.2337-12.2013.
  PUBMED    23536066
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2337-12"; journalName: "J Neurosci"; date: 
            "2013-03-27- 27"; volume: "33"; issue: "13"; pages: "5507-23"
COMMENT     Sequence Label: mCer-Vamp2(qv/fw)
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    508..629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(545..564)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     599..618
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(773..794)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gacgagctgt acaagtccgg actcaccatg tcggctaccg ctgccaccgt cccgcctgcc
       61 gccccggccg gcgagggtgg cccccctgca cctcctccaa atcttaccag taacaggaga
      121 ctgcagcaga cccaggccca ggtggatgag gtggtggaca tcatgagggt gaatgtggac
      181 aaggtcctgg agcgagacca gaagctatcg gaactggatg atcgcgcaga tgccctccag
      241 gcaggggcct ccgtgtggga aacaagtgca gccaagctca agcgcaaata ctggtggaaa
      301 aacctcaaga tgatgatcat cttgggagtg atttgcgcca tcatcctcat catcatcatc
      361 gtttacttca gcacttaagg atccaccgga tctagataac tgatcataat cagccatacc
      421 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
      481 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
      541 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
      601 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt
      661 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
      721 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
      781 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
      841 tcagggcgat ggcccactac gtgaaccatc accctaatca agtttt
//
