LOCUS       sequence-89456-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric 
            Citrine, connected with a 12 amino acid linker. Expressed in 
            mammalian cells, used with Stx1a-12aa-mCer to measure FRET and 
            dispersion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Degtyar V, Hafez IM, Bray C, Zucker RS
  TITLE     Dance of the SNAREs: assembly and rearrangements detected with FRET 
            at neuronal synapses.
  JOURNAL   J Neurosci. 2013 Mar 27;33(13):5507-23. doi: 
            10.1523/JNEUROSCI.2337-12.2013.
  PUBMED    23536066
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2337-12"; journalName: "J Neurosci"; date: 
            "2013-03-27- 27"; volume: "33"; issue: "13"; pages: "5507-23"
COMMENT     Sequence Label: VAMP2-12aa-mCit
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(449..652)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(478..498)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccttgctcac catggtggcg accggtggat ccgcagtgct gaagtaaacg atgatgatga
       61 tgaggatgat ggcgcaaatc actcccaaga tgatcatcat cttgaggttt ttccaccagt
      121 atttgcgctt gagcttggct gcacttgttt caaactggga ggcccctgcc tggagggcat
      181 ctgcgcgatc atccagttcc gatagcttct ggtctcgctc caggaccttg tccacattca
      241 ccctcatgat gtccaccacc tcatccacct gggcctgggt ctgctgcagt ctcctgttac
      301 tggtaagatt tggaggaggt gcaggggggc caccctcgcc ggccggggcg gcaggcggga
      361 cggtggcagc ggtagccgac atgaattcga agcttgagct cgagatctga gtccggtagc
      421 gctagcggat ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac
      481 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
      541 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
      601 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
      661 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
      721 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
      781 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
      841 gtcaatggaa agtccctatt ggcgttacta tggg
//
