LOCUS       sequence-89457-e         576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Cypridina luciferase, puromycin 
            N-acetyl-transferase and shRNAmir of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Charles JP, Fuchs J, Hefter M, Vischedyk JB, Kleint M, Vogiatzi F, 
            Schafer JA, Nist A, Timofeev O, Wanzel M, Stiewe T
  TITLE     Monitoring the dynamics of clonal tumour evolution in vivo using 
            secreted luciferases.
  JOURNAL   Nat Commun. 2014 Jun 3;5:3981. doi: 10.1038/ncomms4981.
  PUBMED    24889111
REFERENCE   2  (bases 1 to 576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-3"; volume: "5:3981. doi"; pages: " 
            10.1038/ncomms4981"
COMMENT     Sequence Label: pCLucIPZ
FEATURES             Location/Qualifiers
     source          1..576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtaccgagct cggatccgcc accatgaaga ccttaattct tgccgttgca ttagtctact
       61 gcgccactgt tcattgccag gactgtcctt acgaacctga tccaccaaac acagttccaa
      121 cttcctgtga agctaaagaa ggagaatgta ttgatagcag ctgtggcacc tgcacgagag
      181 acatactatc agatggactg tgtgaaaata aaccaggaaa aacatgttgc cgaatgtgtc
      241 agtatgtaat tgaatgcaga gtagaggccg caggatggtt tagaacattc tatggaaaga
      301 gattccagtt ccaggaacct ggtacatacg tgttgggtca aggaaccaag ggcggcgact
      361 ggaaggtgtc catcaccctg gagaacctgg atggaaccaa gggggctgtg ctgaccaaga
      421 caagactgga agtggctgga gacatcattg acatcgctca agctactgag aatcccatca
      481 ctgtaaacgg tggagctgac cctatcatcg ccaacccgta caccatcggc gaggtcacca
      541 tcgctgttgt tgagatgcca ggcttcaaca tcaccg
//
