LOCUS       sequence-89463-e         355 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of Cypridina luciferase and shRNAmir of 
            interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Charles JP, Fuchs J, Hefter M, Vischedyk JB, Kleint M, Vogiatzi F, 
            Schafer JA, Nist A, Timofeev O, Wanzel M, Stiewe T
  TITLE     Monitoring the dynamics of clonal tumour evolution in vivo using 
            secreted luciferases.
  JOURNAL   Nat Commun. 2014 Jun 3;5:3981. doi: 10.1038/ncomms4981.
  PUBMED    24889111
REFERENCE   2  (bases 1 to 355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-3"; volume: "5:3981. doi"; pages: " 
            10.1038/ncomms4981"
COMMENT     Sequence Label: pIND-CLucZ
FEATURES             Location/Qualifiers
     source          1..355
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(149..275)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(303..317)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 aggatcagga cgctcgctgc gcccttcgtc tgacgtggca gcgctcgccg tgaggagggg
       61 ggcgcccgcg ggaggcgcca aaacccggcg cggaggccag atcttacgcg tcgccgcgtg
      121 tttaaacgca ttagtcttcc aattgaaaaa agtgatttaa tttataccat tttaattcag
      181 ctttgtaaaa atgtatcaaa gagatagcaa ggtattcagt tttagtaaac aagataattg
      241 ctcctaaagt agccccttga attccgaggc agtaggcact ctcgcttggg cgagagtaag
      301 tacatctgtg gcttcactac ttactctcgc ccaagcgaga tcgctcactg tcaac
//
