LOCUS       sequence-89464-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of Cypridina luciferase and shRNAmir of 
            interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Charles JP, Fuchs J, Hefter M, Vischedyk JB, Kleint M, Vogiatzi F, 
            Schafer JA, Nist A, Timofeev O, Wanzel M, Stiewe T
  TITLE     Monitoring the dynamics of clonal tumour evolution in vivo using 
            secreted luciferases.
  JOURNAL   Nat Commun. 2014 Jun 3;5:3981. doi: 10.1038/ncomms4981.
  PUBMED    24889111
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-3"; volume: "5:3981. doi"; pages: " 
            10.1038/ncomms4981"
COMMENT     Sequence Label: pIND-CLucZ
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    130..163
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
       61 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
      121 gacggatcca taacttcgta taatgtatgc tatacgaagt tatgccacca tgtctagact
      181 ggacaagagc aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg
      241 cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca
      301 cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca
      361 tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa
      421 gtcataccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg
      481 cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg
      541 cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg
      601 cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga
      661 ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc
      721 tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga
      781 aagcggcggg ccgaccgacg cccttgacga
//
