LOCUS       sequence-89468-e         340 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Cypridina luciferase, puromycin 
            N-acetyl-transferase and shRNAmir of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Charles JP, Fuchs J, Hefter M, Vischedyk JB, Kleint M, Vogiatzi F, 
            Schafer JA, Nist A, Timofeev O, Wanzel M, Stiewe T
  TITLE     Monitoring the dynamics of clonal tumour evolution in vivo using 
            secreted luciferases.
  JOURNAL   Nat Commun. 2014 Jun 3;5:3981. doi: 10.1038/ncomms4981.
  PUBMED    24889111
REFERENCE   2  (bases 1 to 340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-3"; volume: "5:3981. doi"; pages: " 
            10.1038/ncomms4981"
COMMENT     Sequence Label: pCLucIPZ
FEATURES             Location/Qualifiers
     source          1..340
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     ncRNA           complement(157..283)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(311..325)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 aattttgtaa tccagaggtt gattgttcca gacgcgtcct aggtaatacg actcactata
       61 gggcccgccc gcaaacaaca tcaaacaaac cagcagacat atgcaacaag acacgagacg
      121 gagtaatggc cggccgcatt agtcttccaa ttgaaaaaag tgatttaatt tataccattt
      181 taattcagct ttgtaaaaat gtatcaaaga gatagcaagg tattcagttt tagtaaacaa
      241 gataattgct cctaaagtag ccccttgaat tccgaggcag taggcactct cgcttgggcg
      301 agagtaagta catctgtggc ttcactactt actctcgccc
//
