LOCUS       sequence-89471-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for Human MBD3 conditional knockout. Also creates 
            hypomorphic alleles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Rais Y, Zviran A, Geula S, Gafni O, Chomsky E, Viukov S, Mansour AA,
            Caspi I, Krupalnik V, Zerbib M, Maza I, Mor N, Baran D, Weinberger 
            L, Jaitin DA, Lara-Astiaso D, Blecher-Gonen R, Shipony Z, Mukamel Z,
            Hagai T, Gilad S, Amann-Zalcenstein D, Tanay A, Amit I, Novershtern 
            N, Hanna JH
  TITLE     Deterministic direct reprogramming of somatic cells to pluripotency.
  JOURNAL   Nature. 2013 Oct 3;502(7469):65-70. doi: 10.1038/nature12587. Epub 
            2013 Sep 18.
  PUBMED    24048479
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12587"; journalName: "Nature"; date: "2013-10-3- 3"; 
            volume: "502"; issue: "7469"; pages: "65-70"
COMMENT     Sequence Label: pNTK human MBD3 PGK-Neo-pA flox
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(518..551)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gctggggtcg ctgtgcgttg agctcacctg gcgcggctgg tccaccacct tctgcgggtc
       61 gctcttgacc ttgttgttgg ggtggttggt aatcttggtc accggctgct tgaagatgga
      121 cgccgtctgg cgcacgggca gcgccgtgtt caggtcgggc ttgccctgcg ggaggaagga
      181 tatgcagtcc ggcctggggg cgccccggcg gcgcggagcc tcagggggtg cggggcccgg
      241 gtgaccccct gctttgccgg cgcccctcgt gtcccccgcg cccgccagga cccccacggt
      301 ccggggaggc cgctcccgca gggtcggtcc ggccggctct ggaacgcccg ccgcgggccg
      361 cgtcctcgcc atgcgccttg cgctctgcac cctgtggcct gcgccttccg ctctgcgtcc
      421 tccgcccgct gtgacctcct gcgctcagga cgccgggctg tgtcgcctgc agctcacgtc
      481 atggccgcgt ccccgcctag aacgccccgc gagatctata acttcgtata gcatacatta
      541 tacgaagtta taagcttccg acgtcacctg cgtgacgcca ccactcacct tgacctggtt
      601 ggaggagtcg tagcgcacgc gctggcggct cttgttcatc ttgctcatca gcatcttgcc
      661 cgtgcggaag tcgaaggtgc tcaggtccat ggaaccgccc aggtagcgcg ccagctgcgg
      721 catgctgcgg aacttcttcc cgctc
//
