LOCUS       sequence-89473-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for Human MBD3 conditional knockout. Also creates 
            hypomorphic alleles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Rais Y, Zviran A, Geula S, Gafni O, Chomsky E, Viukov S, Mansour AA,
            Caspi I, Krupalnik V, Zerbib M, Maza I, Mor N, Baran D, Weinberger 
            L, Jaitin DA, Lara-Astiaso D, Blecher-Gonen R, Shipony Z, Mukamel Z,
            Hagai T, Gilad S, Amann-Zalcenstein D, Tanay A, Amit I, Novershtern 
            N, Hanna JH
  TITLE     Deterministic direct reprogramming of somatic cells to pluripotency.
  JOURNAL   Nature. 2013 Oct 3;502(7469):65-70. doi: 10.1038/nature12587. Epub 
            2013 Sep 18.
  PUBMED    24048479
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12587"; journalName: "Nature"; date: "2013-10-3- 3"; 
            volume: "502"; issue: "7469"; pages: "65-70"
COMMENT     Sequence Label: pNTK human MBD3 PGK-Neo-pA flox
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(52..551)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(100..119)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     493..516
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    572..605
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(622..655)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 actgcgtgca atccatcttg ttcaatggcc gatcccatat tggctgcagg tcgaaaggcc
       61 cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc
      121 cgggcctccg gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc
      181 cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg
      241 gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag
      301 actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg
      361 ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg
      421 agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta
      481 gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc
      541 ctcccctacc cggtagggcg cgccgggatc cgaagttcct attctctaga aagtatagga
      601 acttcatcga tcctagaatt cataacttcg tatagcatac attatacgaa gttatccgcg
      661 gctagacccg ggggggtcga cgaccccagc accccacaac tggcccctag atctgggcgc
      721 cagccagacc cagagcactg gccccgatcc tcacaccctg gccctagccc cagcctccac
      781 atcggatcct tgctccagac ccccaacccc ggtcccctct gaatcctccc
//
