LOCUS       sequence-89477-e        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of human reptin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Venteicher AS, Meng Z, Mason PJ, Veenstra TD, Artandi SE
  TITLE     Identification of ATPases pontin and reptin as telomerase components
            essential for holoenzyme assembly.
  JOURNAL   Cell. 2008 Mar 21;132(6):945-57. doi: 10.1016/j.cell.2008.01.019.
  PUBMED    18358808
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2008.01"; journalName: "Cell"; date: "2008-03-21- 
            21"; volume: "132"; issue: "6"; pages: "945-57"
COMMENT     Sequence Label: pMGIB-3xFLAG-reptin
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     329..345
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     promoter        375..393
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      454..463
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             475..540
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ccttctctag gcgcccccat atgagatctt atatggggca cccccgcccc ttgtaaactt
       61 ccctgaccct gacatgacaa gagttactaa cagcccctct ctccaagctc acttacaggc
      121 tctctactta gtccagcacg aagtctggag acctctggcg gcagcctacc aagaacaact
      181 ggaccgaccg gtggtacctc acccttaccg agtcggcgac acagtgtggg tccgccgaca
      241 ccagactaag aacctagaac ctcgctggaa aggaccttac acagtcctgc tgaccacccc
      301 caccgccctc aaagtagacg gcatcgcagc ttggatacac gccgcccacg taaaacaatt
      361 gaagatctag tcgaaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt
      421 ggcggccaat tcgagctcgg tacccgttta aacgccacca tggggtcccg ggctgactac
      481 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
      541 gggcgcgccg caaccgttac agccacaacc aaagtcccgg agatccgtga tgtaacaagg
      601 attgagcgaa tcggtgccca ctcccacatc cggggactgg ggctggacga tgccttggag
      661 cctcggcagg cttcgcaagg catggtgggt cagctggcgg cacggcgggc ggctggcgtg
      721 gtgctggaga tgatccggga agggaagatt gccggtcggg cagtccttat tgctggccag
      781 ccgggcacgg ggaagacggc catcgccatg ggcatggcgc aggccctggg ccctgacacg
      841 ccattcacag ccatcgccgg cagtgaaatc ttctccctgg agatgagcaa gaccgaggcg
      901 ctgacgcagg ccttccggcg gtccatcggc gttcgcatca aggaggagac ggagatcatc
      961 gaaggggagg tggtggagat ccagattgat cgaccagcaa cagggacggg ctccaaggtg
     1021 ggcaaactga
//
