LOCUS       sequence-89480-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST and HA tagged human ERK2 R67S D321N from bacterial 
            expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pGEX4T3-HA-ERK2 GOF
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(61..96)
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
ORIGIN
        1 caccgaaacg cgcgaggcag atcgtcagtc agtcacgatg cggccgctcg agtcgactta
       61 agatctgtat cctggctgga atctagcagt ctcttcaaaa attagttctt tgagcttttc
      121 cttaggcaag tcatccaatt ccatgtcgaa cttgaatggt gcttcggcga tgggctcgtt
      181 actactcggg tcgtaatact gctccagata tgggtgggcc agagcctgtt ctacttcaat
      241 cctcttgtgt gggttgaatg tcaacatttt gtccaataag tccagagctt tggagtcagc
      301 atttgggaac agcctgttcc atggcacctt atttttgtgt ggaagagaaa gcaaatagtt
      361 cctagctttt aaatttatta tacaattcag gtcttcttgt gatggggatc caagaatacc
      421 caaaatgtgg ttcagctggt caagataatg cttccctgga aagatgggcc tgttagaaag
      481 catttctgcc agaatgcagc ctacagacca aatatcaatg gacttggtgt agcccttgga
      541 attcaacata atttctggag ccctgtacca acgtgtggcc acatattctg tcaggaaccc
      601 tgtgtgatca tggtctggat ctgcaacacg ggccaggcca aagtcacaga tcttgagatc
      661 acaggtggtg ttgagcagca ggttggaagg cttgaggtca cggtgcagaa cgttagctga
      721 atggatatat tttaaccctc tgaggatctg gtagagaaaa tagcagatat ggtcattgct
      781 gaggtgttgt gtcttcaaga gcttgtaaag atctgtttcc atgaggtcct gtactatata
      841 tacatctttc atttgctcga tggttggtgc tcgaataaa
//
