LOCUS       sequence-89483-e        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of human dyskerin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Venteicher AS, Abreu EB, Meng Z, McCann KE, Terns RM, Veenstra TD, 
            Terns MP, Artandi SE
  TITLE     A human telomerase holoenzyme protein required for Cajal body 
            localization and telomere synthesis.
  JOURNAL   Science. 2009 Jan 30;323(5914):644-8. doi: 10.1126/science.1165357.
  PUBMED    19179534
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2009-01-30- 30"; volume: "323"; 
            issue: "5914"; pages: "644-8"
COMMENT     Sequence Label: pMGIB-3xFLAG-dyskerin
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     331..347
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     promoter        377..395
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      456..465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             477..542
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctccttctct aggcgccccc atatgagatc ttatatgggg cacccccgcc ccttgtaaac
       61 ttccctgacc ctgacatgac aagagttact aacagcccct ctctccaagc tcacttacag
      121 gctctctact tagtccagca cgaagtctgg agacctctgg cggcagccta ccaagaacaa
      181 ctggaccgac cggtggtacc tcacccttac cgagtcggcg acacagtgtg ggtccgccga
      241 caccagacta agaacctaga acctcgctgg aaaggacctt acacagtcct gctgaccacc
      301 cccaccgccc tcaaagtaga cggcatcgca gcttggatac acgccgccca cgtaaaacaa
      361 ttgaagatct agtcgaaatt aaccctcact aaagggaaca aaagctggag ctccaccgcg
      421 gtggcggcca attcgagctc ggtacccgtt taaacgccac catggggtcc cgggctgact
      481 acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca
      541 aggggcgcgc cgcggatgcg gaagtaatta ttttgccaaa gaaacataag aagaaaaagg
      601 agcggaagtc attgccagaa gaagatgtag ccgaaataca acacgctgaa gaatttctta
      661 tcaaacctga atccaaagtt gctaagttgg acacgtctca gtggcccctt ttgctaaaga
      721 attttgataa gctgaatgta aggacaacac actatacacc tcttgcatgt ggttcaaatc
      781 ctctgaagag agagattggg gactatatca ggacaggttt cattaatctt gacaagccct
      841 ctaacccctc ttcccatgag gtggtagcct ggattcgacg gatacttcgg gtggagaaga
      901 cagggcacag tggtactctg gatcccaagg tgactggttg tttaatcgtg tgcatagaac
      961 gagccactcg cttggtgaag tcacaacaga gtgcaggcaa agagtatgtg gggattgtcc
     1021 ggctgccaa
//
