LOCUS       sequence-8949-ex        3675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Zhang Y, Nash L, Fisher AL.
  TITLE     A simplified, robust, and streamlined procedure for the production 
            of C. elegans transgenes via recombineering
  JOURNAL   BMC Dev Biol. 2008 Dec 30;8(1):119.
  PUBMED    19116030
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2008-12-30"; volume: "8(1)"; pages: "119"
COMMENT     Sequence Label: pMOD4 galK-GT
FEATURES             Location/Qualifiers
     source          1..3675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             124..147
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        196..243
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     misc_feature    1677..1695
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(1834..1853)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1953..1975
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2013..2031)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2099..2203
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2204..2272,2273..3064)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2422..2441)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     3522..3539
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3605..3623
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 ctgcagatgg caggccttgc gcaacacgat gaagccgtgg acaacaaatt caacaaaacc
       61 ggtatggaaa agagaagatg gaaaaagaat ttcatagccg tctcagcagc caactctaga
      121 atggattaca aggacgatga cgataagatg agtaaaggag aagaactttt cactggagtt
      181 gtcccaagaa ttcctgttga caattaatca tcggcatagt atatcggcat agtataatac
      241 gacaaggtga ggaactaaac ccaggaggca gatcatgagt ctgaaagaaa aaacacaatc
      301 tctgtttgcc aacgcatttg gctaccctgc cactcacacc attcaggcgc ctggccgcgt
      361 gaatttgatt ggtgaacaca ccgactacaa cgacggtttc gttctgccct gcgcgattga
      421 ttatcaaacc gtgatcagtt gtgcaccacg cgatgaccgt aaagttcgcg tgatggcagc
      481 cgattatgaa aatcagctcg acgagttttc cctcgatgcg cccattgtcg cacatgaaaa
      541 ctatcaatgg gctaactacg ttcgtggcgt ggtgaaacat ctgcaactgc gtaacaacag
      601 cttcggcggc gtggacatgg tgatcagcgg caatgtgccg cagggtgccg ggttaagttc
      661 ttccgcttca ctggaagtcg cggtcggaac cgtattgcag cagctttatc atctgccgct
      721 ggacggcgca caaatcgcgc ttaacggtca ggaagcagaa aaccagtttg taggctgtaa
      781 ctgcgggatc atggatcagc taatttccgc gctcggcaag aaagatcatg ccttgctgat
      841 cgattgccgc tcactgggga ccaaagcagt ttccatgccc aaaggtgtgg ctgtcgtcat
      901 catcaacagt aacttcaaac gtaccctggt tggcagcgaa tacaacaccc gtcgtgaaca
      961 gtgcgaaacc ggtgcgcgtt tcttccagca gccagccctg cgtgatgtca ccattgaaga
     1021 gttcaacgct gttgcgcatg aactggaccc gatcgtggca aaacgcgtgc gtcatatact
     1081 gactgaaaac gcccgcaccg ttgaagctgc cagcgcgctg gagcaaggcg acctgaaacg
     1141 tatgggcgag ttgatggcgg agtctcatgc ctctatgcgc gatgatttcg aaatcaccgt
     1201 gccgcaaatt gacactctgg tagaaatcgt caaagctgtg attggcgaca aaggtggcgt
     1261 acgcatgacc ggcggcggat ttggcggctg tatcgtcgcg ctgatcccgg aagagctggt
     1321 gcctgccgta cagcaagctg tcgctgaaca atatgaagca aaaacaggta ttaaagagac
     1381 tttttacgtt tgtaaaccat cacaaggagc aggacagtgc tgaggatccc ttcttgagtt
     1441 tgtaacagct gctgggatta cacatggcat ggacgaacta tacaaaagcc cacaagcttt
     1501 gggtacccta aatggtgctc aggcgccgaa agtagacgcg aattccgcgg ggaagtcaac
     1561 cagtactgcc gtctcagcag ccaaccgctt taagaaaatc tcatcctccg gggcacttga
     1621 gctcgaattc gagccaatat gcgagaacac ccgagaaaat tcatcgatga tggttgagat
     1681 gtgtataaga gacagctgtc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     1741 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     1801 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     1861 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     1921 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     1981 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     2041 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     2101 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2161 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2221 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     2281 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     2341 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     2401 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     2461 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     2521 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     2581 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     2641 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     2701 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     2761 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     2821 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     2881 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     2941 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3001 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3061 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3121 atttgtgcat ccgaagatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt
     3181 ttcacgtact aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc
     3241 tcatggctaa cgtactaagc tctcatggct aacgtactaa gctctcatgg ctaacgtact
     3301 aagctctcat gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg
     3361 ttttaagttt tataagaaaa aaaagaatat ataaggcttt taaagctagc ttttaaggtt
     3421 tcaccatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3481 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3541 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaag
     3601 acagctgtct cttatacaca tctcaaccct gaagctcttg ttggctagtg cgtagtcgtt
     3661 ggcaagcttg catgc
//
