LOCUS       sequence-89499-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST and HA tagged human ERK2 R67S from bacterial 
            expression vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pGEX4T3-HA-ERK2 R67S
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(66..101)
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
ORIGIN
        1 gtcatcaccg aaacgcgcga ggcagatcgt cagtcagtca cgatgcggcc gctcgagtcg
       61 acttaagatc tgtatcctgg ctggaatcta gcagtctctt caaaaattag ttctttgagc
      121 ttttccttag gcaagtcatc caattccatg tcgaacttga atggtgcttc ggcgatgggc
      181 tcgtcactcg ggtcgtaata ctgctccaga tatgggtggg ccagagcctg ttctacttca
      241 atcctcttgt gtgggttgaa tgtcaacatt ttgtccaata agtccagagc tttggagtca
      301 gcatttggga acagcctgtt ccatggcacc ttatttttgt gtggaagaga aagcaaatag
      361 ttcctagctt ttaaatttat tatacaattc aggtcttctt gtgatgggga tccaagaata
      421 cccaaaatgt ggttcagctg gtcaagataa tgcttccctg gaaagatggg cctgttagaa
      481 agcatttctg ccagaatgca gcctacagac caaatatcaa tggacttggt gtagcccttg
      541 gaattcaaca taatttctgg agccctgtac caacgtgtgg ccacatattc tgtcaggaac
      601 cctgtgtgat catggtctgg atctgcaaca cgggccaggc caaagtcaca gatcttgaga
      661 tcacaggtgg tgttgagcag caggttggaa ggcttgaggt cacggtgcag aacgttagct
      721 gaatggatat attttaaccc tctgaggatc tggtagagaa aatagcagat atggtcattg
      781 ctgaggtgtt gtgtcttcaa gagcttgtaa agatctgttt ccatgaggtc ctgtactata
      841 tatacatctt tcatttgctc gatggt
//
