LOCUS       sequence-89503-e        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST and HA tagged human ERK2 D321N from bacterial 
            expression vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pGEX4T3-HA-ERK2 D321N
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..37
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             74..100
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccatcctcca aaatcggatc tggttccgcg tggatccccg aattcccggg tcgacgatat
       61 cgccgccacc atgtacccat acgatgttcc agattacgct gcggcggcgg cggcggcggg
      121 cgcgggcccg gagatggtcc gcgggcaggt gttcgacgtg gggccgcgct acaccaacct
      181 ctcgtacatc ggcgagggcg cctacggcat ggtgtgctct gcttatgata atgtcaacaa
      241 agttcgagta gctatcaaga aaatcagccc ctttgagcac cagacctact gccagagaac
      301 cctgagggag ataaaaatct tactgcgctt cagacatgag aacatcattg gaatcaatga
      361 cattattcga gcaccaacca tcgagcaaat gaaagatgta tatatagtac aggacctcat
      421 ggaaacagat ctttacaagc tcttgaagac acaacacctc agcaatgacc atatctgcta
      481 ttttctctac cagatcctca gagggttaaa atatatccat tcagctaacg ttctgcaccg
      541 tgacctcaag ccttccaacc tgctgctcaa caccacctgt gatctcaaga tctgtgactt
      601 tggcctggcc cgtgttgcag atccagacca tgatcacaca gggttcctga cagaatatgt
      661 ggccacacgt tggtacaggg ctccagaaat tatgttgaat tccaagggct acaccaagtc
      721 cattgatatt tggtctgtag gctgcattct ggcagaaatg ctttctaaca ggcccatctt
      781 tccagggaag cattatcttg accagctgaa ccacattttg ggtattcttg gatccccatc
      841 acaagaagac ctgaattgta taataaattt aaaagctagg aactatttgc tttctcttcc
      901 acacaaaaat aaggtgccat ggaacaggct gttcccaaat gctgactcca aagctctgga
      961 cttattggac aaaatgttga cattcaaccc acacaagagg attgaagtag aacaggctct
     1021 ggcccaccca tatctggagc agtattacga cccgagtagt aacgagccca tcgccgaagc
     1081 accattcaag
//
