LOCUS       sequence-89505-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST and HA tagged human ERK2 D321N from bacterial 
            expression vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pGEX4T3-HA-ERK2 D321N
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(66..101)
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
ORIGIN
        1 gtcatcaccg aaacgcgcga ggcagatcgt cagtcagtca cgatgcggcc gctcgagtcg
       61 acttaagatc tgtatcctgg ctggaatcta gcagtctctt caaaaattag ttctttgagc
      121 ttttccttag gcaagtcatc caattccatg tcgaacttga atggtgcttc ggcgatgggc
      181 tcgttactac tcgggtcgta atactgctcc agatatgggt gggccagagc ctgttctact
      241 tcaatcctct tgtgtgggtt gaatgtcaac attttgtcca ataagtccag agctttggag
      301 tcagcatttg ggaacagcct gttccatggc accttatttt tgtgtggaag agaaagcaaa
      361 tagttcctag cttttaaatt tattatacaa ttcaggtctt cttgtgatgg ggatccaaga
      421 atacccaaaa tgtggttcag ctggtcaaga taatgcttcc ctggaaagat gggcctgtta
      481 gaaagcattt ctgccagaat gcagcctaca gaccaaatat caatggactt ggtgtagccc
      541 ttggaattca acataatttc tggagccctg taccaacgtg tggccacata ttctgtcagg
      601 aaccctgtgt gatcatggtc tggatctgca acacgggcca ggccaaagtc acagatcttg
      661 agatcacagg tggtgttgag cagcaggttg gaaggcttga ggtcacggtg cagaacgtta
      721 gctgaatgga tatattttaa ccctctgagg atctggtaga gaaaatagca gatatggtca
      781 ttgctgaggt gttgtgtctt caagagcttg taaagatctg tttccatgag gtcctgtact
      841 atatatacat ctttcatttg ctcgatggtt ggtgctcg
//
