LOCUS       sequence-89512-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nano-lantern-based luminescent cAMP indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(cAMP-3.3)/pRSETB
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..94
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(98..106,107..121)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(158..186)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     781..801
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttgtttactt taagaaggag atatacatat gcggggttct catcatcatc atcatcatgg
       61 tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa
      121 ggatccgatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      181 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      241 atacggaaaa cttaccctta aattaatttg cactactgga aaactacctg ttccatggcc
      301 aacacttgtc actactttag gttatggtct tcaatgcttt gcgagatacc cagatcatat
      361 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      421 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      481 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      541 acacaaattg gaatacaact ataactcaca caatgtatac atcacagcag acaaacaaaa
      601 gaatggaatc aaagcaaact tcaaaattag acacaacatt gaagatggag gtgttcaact
      661 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
      721 ccattacctg tcctatcaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
      781 ggtccttctt gagtttgtaa cagctgctgg gggtaccaag gtgtacgacc ccgagcagag
      841 gaagaggatg atcaccggcc cccagtggtg ggccaggtgc aagcagatga acgtgctgga
      901 cagcttcatc aactactac
//
