LOCUS       sequence-89520-e        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-HA tagged human MEK5 from bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pGEX6P1-HA-MEK5
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..43
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             65..91
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccatcctcca aaatcggatc tggaagttct gttccagggg cccctgggat ccgccgccac
       61 catgtaccca tacgatgttc cagattacgc tctgtggcta gcccttggcc cctttcctgc
      121 catggagaac caggtgctgg taattcgcat caagatccca aatagtggcg cggtggactg
      181 gacagtgcac tccgggccgc agttactctt cagggatgtg ctggatgtga taggccaggt
      241 tctgcctgaa gcaacaacta cagcatttga atatgaagat gaagatggtg atcgaattac
      301 agtgagaagt gatgaggaaa tgaaggcaat gctgtcatat tattattcca cagtaatgga
      361 acagcaagta aatggacagt taatagagcc tctgcagata tttccaagag cctgcaagcc
      421 tcctggggaa cggaacatac atggcctgaa ggtgaatact cgggccggac cctctcaaca
      481 cagcagccca gcagtctcag attcacttcc aagcaatagc ttaaagaagt cttctgctga
      541 actgaaaaaa atactagcca atggccagat gaatgaacaa gacatacgat atcgggacac
      601 tcttggtcat ggcaacggag gcacagtcta caaagcatat catgtcccga gtgggaaaat
      661 attagctgta aaggtcatac tactagatat tacactggaa cttcagaagc aaattatgtc
      721 tgaattggaa attctttata agtgcgattc atcatatatc attggatttt atggagcatt
      781 ttttgtagaa aacaggattt caatatgtac agaattcatg gatgggggat ctttggatgt
      841 atataggaaa atgccagaac atgtccttgg aagaattgca gtagcagttg ttaaaggcct
      901 tacttatttg tggagtttaa agattttaca tagagacgtg aagccctcca atatgctagt
      961 aaacacaaga ggacaggtta agctgtgtga ttttggagtt agcactcagc tggtgaattc
     1021 tatagccaag acgtatgttg gaacaaatgc ttatatggcg cctgaaagga
//
