LOCUS       sequence-89527-e        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nano-lantern-based luminescent cAMP indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(cAMP-0.4)/pcDNA3
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        31..49
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(117..145)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     740..760
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
       61 ttggtaccga gctcggatcc gccaccatga gtaaaggaga agaacttttc actggagttg
      121 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
      181 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa attaatttgc actactggaa
      241 aactacctgt tccatggcca acacttgtca ctactttagg ttatggtctt caatgctttg
      301 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
      361 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
      421 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
      481 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
      541 tcacagcaga caaacaaaag aatggaatca aagcaaactt caaaattaga cacaacattg
      601 aagatggagg tgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
      661 ctgtcctttt accagacaac cattacctgt cctatcaatc tgccctttcg aaagatccca
      721 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg ggtaccaagg
      781 tgtacgaccc cgagcagagg aagaggatga tcaccggccc ccagtggtgg gccaggtgca
      841 agcagatgaa cgtgctggac agcttcatca actactacga cagcgagaag cacgccgaga
      901 acgccgtgat cttcctgcac ggcaacgcca ctagcagcta cctgtggagg cacgtggtgc
      961 cccacatcga gcccgtggcc aggtgcatca tccccgatct gatcggcatg ggcaagagcg
//
