LOCUS       sequence-89531-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  monomeric photosensitizing fluorescent protein for 
            chromophore-assisted light inactivation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Takemoto K, Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, 
            Arai Y, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H, Nagai T
  TITLE     SuperNova, a monomeric photosensitizing fluorescent protein for 
            chromophore-assisted light inactivation.
  JOURNAL   Sci Rep. 2013;3:2629. doi: 10.1038/srep02629.
  PUBMED    24043132
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013"; volume: "3:2629. doi"; pages: " 10.1038/srep02629"
COMMENT     Sequence Label: SuperNova/pRSETB
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     86..105
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        86..104
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             136..158
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             178..195
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             199..231
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(235..243,244..258)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
       61 atgcaggatc tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt
      121 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgcg gggttctcat
      181 catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg
      241 tacgacgatg acgataagga tcccaccatg ggttcagagg tcggccccgc cctgttccag
      301 agcgacatga ccttcaaaat cttcatcgac ggcgaggtga acggccagaa gttcaccatc
      361 gtggccgacg gcagcagcaa gttcccccac ggcgacttca acgtgcacgc cgtgtgcgag
      421 accggcaagc tgcccatgag ctggaagccc atctgccacc tgatccagta cggcgagccc
      481 ttcttcgccc gctaccccga cggcatcagc catttcgccc aggagtgctt ccccgagggc
      541 ctgagcatcg accgcaccgt gcgcttcgag aacgacggca ccatgaccag ccaccacacc
      601 tacgagctgg acgacacctg cgtggtgagc cgcatcaccg tgaactgcga cggcttccag
      661 cccgacggcc ccatcatgcg cgaccagctg gtggacatcc tgcccagcga gacccacatg
      721 ttcccccacg gccccaacgc cgtgcgccag accgccacca tcggcttcac caccgccgac
      781 ggcggcaaga tgatgggcca cttcgacagc aagatgacct tcaacggcag ccgcgccatc
      841 gagatccccg gcccacactt cgtgaccatc atcaccaagc agacgaggga cactagcgac
      901 aagcgcga
//
