LOCUS       sequence-89533-e        1155 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nano-lantern-based luminescent cAMP indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(cAMP-3.3)/pcDNA3
FEATURES             Location/Qualifiers
     source          1..1155
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        39..57
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(125..153)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     748..768
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
       61 acccaagctt ggtaccgagc tcggatccgc caccatgagt aaaggagaag aacttttcac
      121 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      181 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat taatttgcac
      241 tactggaaaa ctacctgttc catggccaac acttgtcact actttaggtt atggtcttca
      301 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
      361 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
      421 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
      481 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
      541 tgtatacatc acagcagaca aacaaaagaa tggaatcaaa gcaaacttca aaattagaca
      601 caacattgaa gatggaggtg ttcaactagc agaccattat caacaaaata ctccaattgg
      661 cgatggccct gtccttttac cagacaacca ttacctgtcc tatcaatctg ccctttcgaa
      721 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctggggg
      781 taccaaggtg tacgaccccg agcagaggaa gaggatgatc accggccccc agtggtgggc
      841 caggtgcaag cagatgaacg tgctggacag cttcatcaac tactacgaca gcgagaagca
      901 cgccgagaac gccgtgatct tcctgcacgg caacgccact agcagctacc tgtggaggca
      961 cgtggtgccc cacatcgagc ccgtggccag gtgcatcatc cccgatctga tcggcatggg
     1021 caagagcggc aagagcggca acggcagcta caggctgctg gaccactaca agtacctgac
     1081 cgcctggttc gagcttctga acctgcccaa gaagatcatc ttcgtgggcc acgactgggg
     1141 cgccgccctg gcctt
//
