LOCUS       sequence-89534-e        1176 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nano-lantern-based luminescent cAMP indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(cAMP-1.6)/pcDNA3
FEATURES             Location/Qualifiers
     source          1..1176
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(133..161)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     756..776
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccgcca ccatgagtaa aggagaagaa
      121 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      181 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaatta
      241 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttaggttat
      301 ggtcttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
      361 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
      421 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
      481 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
      541 tcacacaatg tatacatcac agcagacaaa caaaagaatg gaatcaaagc aaacttcaaa
      601 attagacaca acattgaaga tggaggtgtt caactagcag accattatca acaaaatact
      661 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccta tcaatctgcc
      721 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
      781 gctgggggta ccaaggtgta cgaccccgag cagaggaaga ggatgatcac cggcccccag
      841 tggtgggcca ggtgcaagca gatgaacgtg ctggacagct tcatcaacta ctacgacagc
      901 gagaagcacg ccgagaacgc cgtgatcttc ctgcacggca acgccactag cagctacctg
      961 tggaggcacg tggtgcccca catcgagccc gtggccaggt gcatcatccc cgatctgatc
     1021 ggcatgggca agagcggcaa gagcggcaac ggcagctaca ggctgctgga ccactacaag
     1081 tacctgaccg cctggttcga gcttctgaac ctgcccaaga agatcatctt cgtgggccac
     1141 gactggggcg ccgccctggc cttccactac gcctac
//
