LOCUS       sequence-89535-e        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-HA tagged human ERK5 K84R from bacterial expression 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pGEX6P1-HA-ERK5 K84R
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..43
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             65..91
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccatcctcca aaatcggatc tggaagttct gttccagggg cccctgggat ccgccgccac
       61 catgtaccca tacgatgttc cagattacgc tgccgagcct ctgaaggagg aagacggcga
      121 ggacggctct gcggagcccc ccgggcccgt gaaggccgaa cccgcccaca ccgctgcctc
      181 tgtagcggcc aagaacctgg ccctgcttaa agcccgctcc ttcgatgtga cctttgacgt
      241 gggcgacgag tacgagatca tcgagaccat aggcaacggg gcctatggag tggtgtcctc
      301 cgcccgccgc cgcctcaccg gccagcaggt ggccatcaga aagatcccta atgctttcga
      361 tgtggtgacc aatgccaagc ggaccctcag ggagctgaag atcctcaagc actttaaaca
      421 cgacaacatc atcgccatca aggacatcct gaggcccacc gtgccctatg gcgaattcaa
      481 atctgtctac gtggtcctgg acctgatgga aagcgacctg caccagatca tccactcctc
      541 acagcccctc acactggaac acgtgcgcta cttcctgtac caactgctgc ggggcctgaa
      601 gtacatgcac tcggctcagg tcatccaccg tgacctgaag ccctccaacc tattggtgaa
      661 tgagaactgt gagctcaaga ttggtgactt tggcatggct cgtggcctgt gcacctcgcc
      721 cgctgaacat cagtacttca tgactgagta tgtggccacg cgctggtacc gtgcgcccga
      781 gctcatgctc tctttgcatg agtatacaca ggctattgac ctctggtctg tgggctgcat
      841 ctttggtgag atgctggccc ggcgccagct cttcccaggc aaaaactatg tacaccagct
      901 acagctcatc atgatggtgc tgggtacccc atcaccagcc gtgattcagg ctgtgggggc
      961 tgagagggtg cgggcctata tccagagctt gccaccacgc cagcctgtgc cctgggg
//
