LOCUS       sequence-89540-e        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from 
            bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pGEX6P1-HA-MEK1 CBM
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..43
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             71..97
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccatcctcca aaatcggatc tggaagttct gttccagggg cccctgggat ccgatatcgc
       61 cgccaccatg tacccatacg atgttccaga ttacgctccc aagaagaagc cgacgcccat
      121 ccagctgaac ccggcccccg acggctctgc agttaacggg accagctctg cggagaccaa
      181 cttggaggcc ttgcagaaga agctggagga gctagagctt gatgagcagc agcgaaagcg
      241 ccttgaggcc tttcttaccc agaagcagaa ggtgggagaa ctgaaggatg acgactttga
      301 gaagatcagt gagctggggg ctggcaatgg cggtgtggtg ttcaaggtct cccacaagcc
      361 ttctggcctg gtcatggcca gaaagctaat tcatctggag atcaaacccg caatccggaa
      421 ccagatcata agggagctgc aggttctgca tgagtgcaac tctccgtaca tcgtgggctt
      481 ctatggtgcg ttctacagcg atggcgagat cagtatctgc atggagcaca tggatggagg
      541 ttctctggat caagtcctga agaaagctgg aagaattcct gaacaaattt taggaaaagt
      601 tagcattgct gtaataaaag gcctgacata tctgagggag aagcacaaga tcgcggccag
      661 agatgtcaag ccctccaaca tcctagtcaa ctcccgtggg gagatcaagc tctgtgactt
      721 tggggtcagc gggcagctca tcgactccat ggccaactcc ttcgtgggca caaggtccta
      781 cgcgtcgcca gaaagactcc aggggactgc ttactctgtg cagtcagaca tctggagcat
      841 gggactgtct ctggtagaga tggcggttgg gaggtatccc atccctcctc cagatgccaa
      901 ggagctggag ctgatgtttg ggtgccaggt ggaaggagat gcggctgaga ccccacccag
      961 gccaaggacc cccgggaggc cccttagctc atacggaatg gacagccgac ctcccatggc
     1021 aatttttgag ttgttggatt acatagtcaa cgagcctcct ccaaaactgc ccagtggagt
     1081 gttcagtctg gaatttcaag atttt
//
