LOCUS       sequence-89555-e        2517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY cloning compatible shRNA expression vector; H1 RNA gene 
            promoter; Kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pENTR-H1
FEATURES             Location/Qualifiers
     source          1..2517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(29..128)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(240..259)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             251..1060
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(318..337)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1150..1738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1639..1658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1903..1989
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2081..2108
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     2116..2139
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    2158..2257
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2302..2516)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(2362..2381)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 aattcgcggc cgcactcgag atatctagac ccagctttct tgtacaaagt tggcattata
       61 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
      121 attatttgcc atccagctgc agctctggcc cgtgtctcaa aatctctgat gttacattgc
      181 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
      241 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
      301 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
      361 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
      421 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
      481 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
      541 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
      601 tattgttgat gcgctggcag tgtccctgcg ccggttgcat tcgattcctg tttgtaattg
      661 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
      721 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
      781 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
      841 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
      901 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
      961 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     1021 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     1081 gtaacattat tcagattggg ccccgttcca ctgagcgtca gacccggtag aaaagatcaa
     1141 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1201 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1261 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1321 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1381 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1441 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1501 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1561 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1621 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1681 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1741 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1801 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
     1861 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     1921 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     1981 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
     2041 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
     2101 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
     2161 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
     2221 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
     2281 ggtatcgata agcttagatc tgtggtctca tacagaactt ataagattcc caaatccaaa
     2341 gacatttcac gtttatggtg atttcccaga acacatagcg acatgcaaat attgcagggc
     2401 gccactcccc tgtccctcac agccatcttc ctgccagggc gcacgcgcgc tgggtgttcc
     2461 cgcctagtga cactgggccc gcgattcctt ggagcgggtt gatgacgtca gcgttcg
//
