LOCUS       sequence-89577-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nano-lantern-based luminescent cAMP indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(cAMP-0.4)/pRSETB
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..39
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..75
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(79..87,88..102)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(139..167)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     762..782
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gatatacata tgcggggttc tcatcatcat catcatcatg gtatggctag catgactggt
       61 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatccgat gagtaaagga
      121 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      181 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      241 aaattaattt gcactactgg aaaactacct gttccatggc caacacttgt cactacttta
      301 ggttatggtc ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
      361 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      421 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      481 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
      541 tataactcac acaatgtata catcacagca gacaaacaaa agaatggaat caaagcaaac
      601 ttcaaaatta gacacaacat tgaagatgga ggtgttcaac tagcagacca ttatcaacaa
      661 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcctatcaa
      721 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
      781 acagctgctg ggggtaccaa ggtgtacgac cccgagcaga ggaagaggat gatcaccggc
//
