LOCUS       sequence-89578-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replaces the psbA2 gene in Synechocystis with the Ptrc 
            promoter-T7-g10-PHLS gene .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Formighieri C, Melis A
  TITLE     Regulation of beta-phellandrene synthase gene expression, 
            recombinant protein accumulation, and monoterpene hydrocarbons 
            production in Synechocystis transformants.
  JOURNAL   Planta. 2014 Aug;240(2):309-24. doi: 10.1007/s00425-014-2080-8. Epub
            2014 May 20.
  PUBMED    24838596
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00425-014-2080-8"; journalName: "Planta"; date: "2014-08";
            volume: "240"; issue: "2"; pages: "309-24"
COMMENT     Sequence Label: psbA2-Ptrc-T7-g10-PHLS (e)
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(354..359,360..376,377..383)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    392..408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttcagggata tcaataagta ttaggtatat ggatcataat tgtatgcccg actattgctt
       61 aaactgactg accactgacc ttaagagtaa tggcgtgcaa ggcccagtga tcaatttcat
      121 tatttttcat tatttcatct ccattgtccc tgaaaatcag ttgtgtcgcc cctctacaca
      181 gcccagaact atggtaaagg cgcacgaaaa accgccaggt aaactcttct caacccccaa
      241 aacgccctct gtttacccat ggaaaaaacg acaattacaa gaaagtaaaa cttatgtcat
      301 ctataagctt cgtgtatatt aacttcctgt tacaaagatt ctgaaatgag ctgttgacaa
      361 ttaatcatcc ggctcgtata atgtgtggaa attgtgagcg gataacaatt tcatgttaac
      421 tttaagaagg agatatagtt atggctagcc atatgtgtag tttgcaagtt tctgatccta
      481 ttcctaccgg acgccgttcc ggtggttatc ccccggcctt atgggatttc gatactattc
      541 aatccctgaa taccgaatat aagggcgaac gtcacatgcg tcgggaagaa gacttaattg
      601 gtcaagttcg ggaaatgttg gtgcacgaag tagaagatcc cactccccag ttggaattca
      661 ttgacgatct gcataaattg ggcatttcct gccattttga aaacgagatt ctgcaaattc
      721 tcaaatccat ttatctcaac caaaactata aacgggacct ctattctacc agtttagcct
      781 tccgtctctt gcgtcaatac gggtttatct
//
