LOCUS       sequence-89585-e        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Gjoerup O, Zaveri D, Roberts TM
  TITLE     Induction of p53-independent apoptosis by simian virus 40 small t 
            antigen.
  JOURNAL   J Virol. 2001 Oct . 75(19):9142-55.
  PUBMED    11533178
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2001 Oct . 75(19):9142-55."
COMMENT     Sequence Label: pcDNA3 flag p53
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga catggactac aaggacgacg
      121 atgacaaggg atccgaggag ccgcagtcag atcctagcgt cgagccccct ctgagtcagg
      181 aaacattttc agacctatgg aaactacttc ctgaaaacaa cgttctgtcc cccttgccgt
      241 cccaagcaat ggatgatttg atgctgtccc cggacgatat tgaacaatgg ttcactgaag
      301 acccaggtcc agatgaagct cccagaatgc cagaggctgc tccccccgtg gcccctgcac
      361 cagcagctcc tacaccggcg gcccctgcac cagccccctc ctggcccctg tcatcttctg
      421 tcccttccca gaaaacctac cagggcagct acggtttccg tctgggcttc ttgcattctg
      481 ggacagccaa gtctgtgact tgcacgtact cccctgccct caacaagatg ttttgccaac
      541 tggccaagac ctgccctgtg cagctgtggg ttgattccac acccccgccc ggcacccgcg
      601 tccgcgccat ggccatctac aagcagtcac agcacatgac ggaggttgtg aggcgctgcc
      661 cccaccatga gcgctgctca gatagcgatg gtctggcccc tcctcagcat cttatccgag
      721 tggaaggaaa tttgcgtgtg gagtatttgg atgacagaaa cacttttcga catagtgtgg
      781 tggtgcccta tgagccgcct gaggttggct ctgactgtac caccatccac tacaactaca
      841 tgtgtaacag ttcctgcatg ggcggcatga accggaggcc catcctcacc atcatcacac
      901 tggaagactc cagtggtaat ctactgggac ggaacagctt tgaggtgcgt gtttgtgcct
      961 gtcctgggag agaccggcgc acagaggaag agaatctccg caagaaaggg gagcctcacc
     1021 acgagctgcc cccagggagc actaagcgag cactgcccaa caacaccagc t
//
