LOCUS       sequence-89589-e         460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a 
            Cre-dependent virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Stachniak TJ, Ghosh A, Sternson SM
  TITLE     Chemogenetic synaptic silencing of neural circuits localizes a 
            hypothalamus-->midbrain pathway for feeding behavior.
  JOURNAL   Neuron. 2014 May 21;82(4):797-808. doi: 
            10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24.
  PUBMED    24768300
REFERENCE   2  (bases 1 to 460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.04.008"; journalName: "Neuron"; date: 
            "2014-05-21- 21"; volume: "82"; issue: "4"; pages: "797-808"
COMMENT     Sequence Label: AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D
FEATURES             Location/Qualifiers
     source          1..460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..33
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    96..129
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taacttcgta taaagtatcc tatacgaagt tatatcaaaa taggaagacc aatgcttcac
       61 catcgacccg aattgccaag catcaccatc gacccataac ttcgtataat gtatgctata
      121 cgaagttata ctagtaacct acctggcagt gccgatgttc cgatactggc acagcagcag
      181 gtgccggaag gtctttttaa aggtggcgtt gcacagagca tagcaggcag ggttgatggt
      241 gctgttgacg tagcagagcc agtagccaat ggaccacacc gtgtcaggga tgcagctctg
      301 gcagaaggtg ttcaccagga ccatgacgtt gtagggcgtc caggtgagga tgaaggctag
      361 cagaatggca aagatcgttc gtgtcacttt gcgctcccgg gccgccatct gccgcttctt
      421 gcgcacctgg ttgcgagcga tgctggcgaa cttgcgggcc
//
