LOCUS       sequence-89590-e        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human DNMT3A with myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Chen ZX, Mann JR, Hsieh CL, Riggs AD, Chedin F
  TITLE     Physical and functional interactions between the human DNMT3L 
            protein and members of the de novo methyltransferase family.
  JOURNAL   J Cell Biochem. 2005 Aug 1;95(5):902-17.
  PUBMED    15861382
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biochem."; date: "2005-08-1"; volume: "95(5)"; pages: "902-17"
COMMENT     Sequence Label: pcDNA3/Myc-DNMT3A
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..131
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttgccaccat ggagcagaag ctgatctcag
      121 aggaggacct ggaattcatg cccgccatgc cctccagcgg ccccggggac accagcagct
      181 ctgctgcgga gcgggaggag gaccgaaagg acggagagga gcaggaggag ccgcgtggca
      241 aggaggagcg ccaagagccc agcaccacgg cacggaaggt ggggcggcct gggaggaagc
      301 gcaagcaccc cccggtggaa agcggtgaca cgccaaagga ccctgcggtg atctccaagt
      361 ccccatccat ggcccaggac tcaggcgcct cagagctatt acccaatggg gacttggaga
      421 agcggagtga gccccagcca gaggagggga gccctgctgg ggggcagaag ggcggggccc
      481 cagcagaggg agagggtgca gctgagaccc tgcctgaagc ctcaagagca gtggaaaatg
      541 gctgctgcac ccccaaggag ggccgaggag cccctgcaga agcgggcaaa gaacagaagg
      601 agaccaacat cgaatccatg aaaatggagg gctcccgggg ccggctgcgg ggtggcttgg
      661 gctgggagtc cagcctccgt cagcggccca tgccgaggct caccttccag gcgggggacc
      721 cctactacat cagcaagcgc aagcgggacg agtggctggc acgctggaaa agggaggctg
      781 agaagaaagc caaggtcatt gcaggaatga atgctgtgga agaaaaccag gggcccgggg
      841 agtctcagaa ggtggaggag gccagccctc ctgctgtgca gcagcccact gaccccgcat
      901 cccccactgt ggctaccacg cctgagcccg tggggtccga tgctggggac aagaatgcca
      961 ccaaagcagg cgatgacgag ccagagtacg aggacggccg gggctttggc attggggagc
     1021 tggtgtgggg gaaactgcgg ggcttctcct ggtggccagg ccgcattgtg tcttggtgga
     1081 tgac
//
