LOCUS       sequence-89593-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To fuse your protein of interest to the N-terminus of BirA(R118G) 
            and use in proximity-dependent biotin identification (BioID).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  TITLE     Karl Kramer plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: BirA(R118G)-HA destination vector
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(131..255)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
       61 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagatct
      121 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
      181 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
      241 cttgtacaaa gtggttgata accggtcgta cgtctccgga ttcgaattcg gatccaagga
      301 caacaccgtg cccctgaagc tgatcgccct gctggccaac ggcgagttcc actctggcga
      361 gcagctggga gagaccctgg gaatgagcag agccgccatc aacaagcaca tccagacact
      421 gagagactgg ggagtggacg tgttcaccgt gcctggcaag ggctacagcc tgcctgagcc
      481 tatccagctg ctgaacgcca agcagatcct gggacagctg gatggcggaa gcgtggccgt
      541 gctgcctgtg atcgactcca ccaatcagta cctgctggac agaatcggag agctgaagtc
      601 cggcgacgcc tgcatcgccg agtaccagca ggctggcaga ggaggcagag gacggaagtg
      661 gttcagccca ttcggagcca acctgtacct gtccatgttc tggagactgg agcagggacc
      721 tgctgctgcc atcggactga gtctggtgat cggaatcgtg atggccgagg tgctgagaaa
      781 gctgggagcc gacaaggtga gagtgaagtg gcctaatgac ctgtacctcc aggaccgcaa
      841 gctggctggc atcctggtgg agctgacagg caagacaggc gatgccgctc agatcgtgat
      901 cggagccgga atcaacatgg ccatga
//
