LOCUS       sequence-89595-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FRT5-flanked Neo; homologous recombination targeting 
            vector cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: 
            pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    108..332
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     321..338
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(365..385)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcactatagg gcgaattggg tacaggcgcg ccccttacta ggcggccgcg aagttcctat
       61 accttttgaa gaataggaac ttcggaatag gaacttctct aggacgtcca tagagcccac
      121 cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac
      181 cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta
      241 ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca
      301 aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcgag ctctagatct
      361 atcacacttt ccgctttttc ttaggggcgg ccacgtcctc ggtgccgtcc tgggcgtcat
      421 cctcgtcgtc gtcggtggga ggcttggccc aggtgatgct ggctctcttc tcgatggggg
      481 tgccctggcc cctgccggtg gtgctcttgg tcaccacgat cttatccacg aacaggccca
      541 cgaacaccct cttgtcgtcc acgctggctc tgccccacca ggacttgggg ccggtggggt
      601 cggcgtcggc atcctcgggg aaccactgat ccaggggcag cttaggggcc tcggcggcct
      661 ccagctcggc cagtctttcc tcggcgccct gctgtctcag ggtcagggcg gcctgctgct
      721 ttctgaagtg cttcctgccc acagggccgt cataggctcc ggcggctctg tcctcgtaca
      781 gctcctccag ggcgttcagg gcgtcggctc tctcggccac caggttggcc ctctcgccgc
      841 tcttctcggg ggcctcggtc agcttgccga atcttctggc ggcctcccac agcagggcca
      901 gggtctcctc gtcgccctcg gcgtgccgga tcttgttgaa gatccgctcg gccacgaact
      961 tgtc
//
