LOCUS       sequence-89596-e        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of WTAP (Wilms tumor 1 associated protein) in human 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Liu J, Yue Y, Han D, Wang X, Fu Y, Zhang L, Jia G, Yu M, Lu Z, Deng 
            X, Dai Q, Chen W, He C
  TITLE     A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine
            methylation.
  JOURNAL   Nat Chem Biol. 2014 Feb;10(2):93-5. doi: 10.1038/nchembio.1432. Epub
            2013 Dec 6.
  PUBMED    24316715
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1432"; journalName: "Nat Chem Biol"; date: 
            "2014-02"; volume: "10"; issue: "2"; pages: "93-5"
COMMENT     Sequence Label: pcDNA3/Flag-WTAP
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttggtaccg agctcggatc catggattac aaggatgacg
      121 atgacaagac caacgaagaa cctcttccca agaaggttcg attgagtgaa acagacttca
      181 aagttatggc aagagatgag ttaattctaa gatggaaaca atatgaagca tatgtacaag
      241 ctttggaggg caagtacaca gatcttaact ctaatgatgt aactggccta agagagtctg
      301 aagaaaaact aaagcaacaa cagcaggagt ctgcacgcag ggaaaacatc cttgtaatgc
      361 gactagcaac caaggaacaa gagatgcaag agtgtactac tcaaatccag tacctcaagc
      421 aagtccagca gccgagcgtt gcccaactga gatcaacaat ggtagaccca gcgatcaact
      481 tgtttttcct aaaaatgaaa ggtgaactgg aacagactaa agacaaactg gaacaagccc
      541 aaaatgaact gagtgcctgg aagtttacgc ctgatagcca aacagggaaa aagttaatgg
      601 cgaagtgtcg aatgcttatc caggagaatc aagagcttgg aaggcagctg tcccagggac
      661 gtattgcaca acttgaagca gagttggctt tacagaagaa atacagtgag gagcttaaaa
      721 gcagtcagga tgaactgaat gacttcatca tccagcttga tgaagaagta gagggtatgc
      781 agagtaccat tctagttctg cagcagcagc tgaaggagac acgccagcag ttggctcagt
      841 accagcagca gcagtctcag gcctctgccc caagtaccag caggactaca gcttctgaac
      901 ctgtagaaca gtcagaggcc acaagtaaag actgcagtcg tctgacaaac ggaccaagta
      961 atggtagctc ctcccgccag aggacgtctg ggtctggatt tcacagggag ggcaacacaa
     1021 ccgaagatga ctttccttct tctccaggga atggtaataa gtcctccaac agctcagagg
//
