LOCUS       sequence-89599-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human 6His-tagged SUMO2 and SUMO-E1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSA2
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        115..133
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    134..158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             214..231
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             253..531
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 2"
                     /label=SUMO2
                     /translation="MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMK
                     AYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     promoter        641..659
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    660..684
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
       61 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aaattaatac
      121 gactcactat aggggaattg tgagcggata acaattcccc tgtagaaata attttgttta
      181 actttaataa ggagatatac catgggcagc agccatcacc atcatcacca cagccaggat
      241 ctggttccgc gtatggccga cgaaaagccc aaggaaggag tcaagactga gaacaacgat
      301 catattaatt tgaaggtggc ggggcaggat ggttctgtgg tgcagtttaa gattaagagg
      361 catacaccac ttagtaaact aatgaaagcc tattgtgaac gacagggatt gtcaatgagg
      421 cagatcagat tccgatttga cgggcaacca atcaatgaaa cagacacacc tgcacagttg
      481 gaaatggagg atgaagatac aattgatgtg ttccaacagc agacgggagg ttaggaattc
      541 atcgtgactg actgacgctg caggtcgaca agcttgcggc cgcataatgc ttaagtcgaa
      601 cagaaagtaa tcgtattgta cacggccgca taatcgaaat taatacgact cactataggg
      661 gaattgtgag cggataacaa ttccccatct tagtatatta gttaagtata agaaggagat
      721 atacatatgg cagatctcaa ttcccgggtc gacatggcac tgtcgcgggg gctgccccgg
      781 gagctggctg aggcggtggc cgggggccgg gtgctggtgg tgggggcggg cggcatcggc
      841 tgcgagctcc tcaagaatct cgtgctcacc
//
