LOCUS       sequence-89600-e        1172 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of METTL3 (methyltransferase-like 3) in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Liu J, Yue Y, Han D, Wang X, Fu Y, Zhang L, Jia G, Yu M, Lu Z, Deng 
            X, Dai Q, Chen W, He C
  TITLE     A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine
            methylation.
  JOURNAL   Nat Chem Biol. 2014 Feb;10(2):93-5. doi: 10.1038/nchembio.1432. Epub
            2013 Dec 6.
  PUBMED    24316715
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1432"; journalName: "Nat Chem Biol"; date: 
            "2014-02"; volume: "10"; issue: "2"; pages: "93-5"
COMMENT     Sequence Label: pcDNA3/Flag-METTL3
FEATURES             Location/Qualifiers
     source          1..1172
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             89..112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttatgga ttacaaggat gacgatgaca agtcggacac
      121 gtggagctct atccaggccc acaagaagca gctggactct ctgcgggaga ggctgcagcg
      181 gaggcggaag caggactcgg ggcacttgga tctacggaat ccagaggcag cattgtctcc
      241 aaccttccgt agtgacagcc cagtgcctac tgcacccacc tctggtggcc ctaagcccag
      301 cacagcttca gcagttcctg aattagctac agatcctgag ttagagaaga agttgctaca
      361 ccacctctct gatctggcct taacattgcc cactgatgct gtgtccatct gtcttgccat
      421 ctccacgcca gatgctcctg ccactcaaga tggggtagaa agcctcctgc agaagtttgc
      481 agctcaggag ttgattgagg taaagcgagg tctcctacaa gatgatgcac atcctactct
      541 tgtaacctat gctgaccatt ccaagctctc tgccatgatg ggtgctgtgg cagaaaagaa
      601 gggccctggg gaggtagcag ggactgtcac agggcagaag cggcgtgcag aacaggactc
      661 gactacagta gctgcctttg ccagttcgtt agtctctggt ctgaactctt cagcatcgga
      721 accagcaaag gagccagcca agaaatcaag gaaacatgct gcctcagatg ttgatctgga
      781 gatagagagc cttctgaacc aacagtccac taaggaacaa cagagcaaga aggtcagtca
      841 ggagatccta gagctattaa atactacaac agccaaggaa caatccattg ttgaaaaatt
      901 tcgctctcga ggtcgggccc aagtgcaaga attctgtgac tatggaacca aggaggagtg
      961 catgaaagcc agtgatgctg atcgaccctg tcgcaagctg cacttcagac gaattatcaa
     1021 taaacacact gatgagtctt taggtgactg ctctttcctt aatacatgtt tccacatgga
     1081 tacctgcaag tatgttcact atgaaattga tgcttgcatg gattctgagg cccctggcag
     1141 caaagaccac acgccaagcc aggaacttgg tc
//
